Fundamentals of Spectrophotometry

Optical spectroscopy in the ultraviolet and visible (UV/VIS) light spectrum is one of the most common techniques for the physical characterization of fluids in a laboratory environment. Based on the absorption of light by a sample, it is widely used across a broad range of fields and applications in research, production and quality control.  

UV/VIS spectrophotometry is frequently used as a rapid non-contact analytical technique for characterizing of both pure substances and fluid mixtures. Used either alone or in combination with other physical analysis methods – such as melting point, refractive index and density measurements – it can provide valuable information about the purity or composition of a sample.

This handy guide provides a straightforward explanation of the fundamentals of UV/VIS spectrophotometry, as well as tips to help you ensure robust and accurate results for routine spectrophotometry applications. It covers:

  • UV/VIS spectroscopy theory
  • The use of UV/VIS spectroscopy in analytical chemistry
  • Basics of spectrophotometer design
  • Industry-specific applications
  • Hints and tips for accurate and precise UV/VIS measurements