In-line Control of CO2 Accumulation for Improved Cell Culture

Controlling dissolved CO₂ measurement in mammalian cell culture processes is growing in acceptance.

dCO₂ concentration in the human body is 5% and when growing animal cells ex-vivo it is necessary to maintain this level as a minimum concentration. In an ex-vivo culture model, for example in a bioreactor, measuring and monitoring dCO2 is considered critical for several reasons. dCO2 accumulation can have a substantial negative effect on cell growth as well as on productivity and the glycosylation of the targeted protein of interest.