Eliminate Unstable Oxygen Measurements for Better Bioreactor Productivity
Dissolved oxygen measurement is essential in fermentation and cell culture for ensuring conditions remain optimal for cells, but air bubbles can create noisy measurements that make it difficult to get an accurate measurement. This white paper discusses how to eliminate the noise that bubbles create, and includes the case study of a large biotech company that improved their fermentation productivity.
As explained in the white paper, low dissolved oxygen levels in fermenters/bioreactors can impact growth rate, nutrients uptake, cellular morphology and metabolite synthesis; leading to reduced yield and lower end-product quality. High levels cause the formation of reactive oxygen species that can oxidize components in the medium and result in cell mutations. Further, on large fermenters that require powerful compressors to inject air, excessive sparging is a costly waste of energy. Maintaining dissolved oxygen in the required range is therefore critical for process optimization.
Accurate oxygen control is only possible if measurements from dissolved oxygen sensors installed in fermenters/bioreactors are reliable. Air bubbles that collect or cross over a sensor's measurement tip create noise on the sensor signal. Erroneous measurement peaks or troughs can lead to inappropriate adjustments to sparging or supplement feeding, compounding issues around fermentation/cell culture control strategies.