Contaminant-Free Pipette Tips for COVID-19 Studies

With COVID-19 currently under intense scrutiny in the pharmaceutical sector, liquid handling has never been more important. Contaminant-free pipette tips are an essential first step to ensuring that both patient diagnostics and studies of the SARS-CoV-2 virus yield accurate, reproducible data.

In recent months, the SARS-CoV-2 novel coronavirus has spread throughout the world from its likely point of origin. The resulting COVID-19 disease was officially declared a pandemic by the World Health Organization on 11 March 2020; as of early April, more than a million cases have been reported globally, and measures to limit contagion are in effect in many countries.

Researchers in industry and academia alike have turned their focus to combatting the pathogen, a single-stranded RNA virus. Key areas of investigation are the development and scale-up of sensitive and specific diagnostic kits, the identification of possible chemotherapeutics via screening of existing compound libraries, and the search for a vaccine.

Intact viral RNA is a prerequisite for the current diagnostic nucleic acid amplification tests, yet pipette tips with trace amounts of biological contamination are a potential – and hidden – source of error, producing false positive and/or false negative results. A white paper outlines best practice for the manufacture and testing of contaminant-free tips, including:

  • ensuring sub-picogram levels of human or bacterial DNA via qPCR analysis
  • using fluoresence based assays for high sensitivity detection of DNase and RNase
  • and controlling for PCR inhibitors.

Download the white paper to learn more about a preventable source of errors and make sure your experiments aren't at risk.